Upcoming events
Stay informed of the latest news of NSure

Scientific publication on NSure molecular test

In March of 2012 an article about ColdNSure and GrowNSure was published in the “New Forrest”, a scientific journal on biology, biotechnology and management of afforestation and reforestation. The following is the abstract of this article:

In boreal forest regions, a great portion of forest tree seedlings are stored indoors in late autumn to prevent seedlings from outdoor winter damage. For seedlings to be able to survive in storage it is crucial that they store well and can cope with the dark and cold storage environment. The aim of this study was to search for genes that can determine the vitality status of Norway spruce (Picea abies (L.) Karst.) seedlings during frozen storage. Furthermore, the sensitivity of the ColdNSureTM test, a gene activity test that predicts storability was assessed. The storability of seedlings was tested biweekly by evaluating damage with the gene activity test and the electrolyte leakage test after freezing seedlings to -25°C (the SELdiff-25 method). In parallel, seedlings were frozen stored at -3 °C. According to both methods, seedlings were considered storable from week 41. This also corresponded to the post storage results determined at the end of the storage period.

In order to identify vitality indicators, Next Generation Sequencing (NGS) was performed on bud samples collected during storage. Comparing physiological post storage data to gene analysis data revealed numerous vitality related genes. To validate the results, a second trial was performed. In this trial, gene activity was better in predicting seedling storability than the conventional freezing test; this indicates a high sensitivity level of this molecular assay. For multiple indicators a clear switch between damaged and vital seedlings was observed. A collection of indicators will be used in the future development of a commercial vitality test.

Downloads
Scientific publication on NSure molecular test (hardiness/vitality) (PDF-document)
Newsletter ColdNSure_0912 (PDF-document)